Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1245543 | Talanta | 2008 | 5 Pages |
Abstract
The chiral resolution of three beta-blockers including propranolol, pindolol and oxprenolol, was studied by affinity electrokinetic chromatography. The effect of various chiral selectors and some key parameters including buffer pH, buffer concentration, capillary temperature and applied voltage were carefully studied, respectively. At optimum condition, based on the signal-to-noise ratio of 3, the detection limits for the simple resolution and chiral resolution were found to be 1.0Â ÃÂ 10â5 and 4.0Â ÃÂ 10â5Â M, respectively. In addition, the interactions of these beta-blockers with bovine serum albumin (BSA) were studied and the binding constant (Ka) between BSA and each of beta-blockers were calculated. Based on linear correlation coefficient, it can be concluded that the binding ratio of pindolol (oxprenolol) combining with BSA is 1:1, and that the binding number of propranolol interacting with BSA deviates one.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Guoqiang Yang, Yifang Zhao, Meixian Li, Zhiwei Zhu, Qiankun Zhuang,