Article ID Journal Published Year Pages File Type
1245547 Talanta 2008 7 Pages PDF
Abstract

The aim of this work is to propose a biomonitoring method for the simultaneous determination of Cd and Pb in whole blood by simultaneous electrothermal atomic absorption spectrometry for assessment of environmental levels. A volume of 200 μL of whole blood was diluted in 500 μL of 0.2% (w v−1) Triton® X-100 + 2.0% (v v−1) HNO3. Trichloroacetic acid was added for protein precipitation and the supernatant analyzed. A mixture of 250 μg W + 200 μg Rh as permanent and 2.0% (w v−1) NH4H2PO4 as co-injected modifiers were used. Characteristic masses and limits of detections (n = 20, 3 s) for Cd and Pb were 1.26 and 33 pg and 0.026 μg L−1 and 0.65 μg L−1, respectively. Repeatability ranged from 1.8 to 6.8% for Cd and 1.2 to 1.7% for Pb. The trueness of method was checked by the analysis of three Reference Materials: Lyphocheck® Whole Blood Metals Control level 1 and Seronorm™ Trace Elements in Whole Blood levels 1 and 2. The found concentrations presented no statistical differences at the 95% confidence level. Blood samples from 40 volunteers without occupational exposure were analyzed and the concentrations ranged from 0.13 to 0.71 μg L−1 (0.32 ± 0.19 μg L−1) for Cd and 9.3 to 56.7 μg L−1 (25.1 ± 10.8 μg L−1) for Pb.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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