On the chemical identification and determination of flavonoids in solid-state

The paper presented with qualitative and quantitative analysis of alkaloids in solid-state, using the excitations within the THz-spectroscopic region of 300–30 cm−1 (9.00–0.9 THz). Series of nine plant flavonoids (FLs) and their mixtures were analyzed both qualitatively and quantitatively. For first time in the literature was reported the quantitative analysis of alkaloids and their mixtures within the THz-region using the solid-state Raman spectroscopy, and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS). The calibration and validation, concentration limit of detection (LOD), limit of quantification (LOQ), and linearity limit (LL) were obtained. The chemometric nonlinear and linear approaches for analysis and interpretation of the quantities were applied. The results obtained were compared with a parallel QA, using the calibrated and validated HPLC electrospray ionization (ESI) mass spectrometric method, electronic absorption (EAs) and CD spectroscopy. The metrology, including accuracy, measurement repeatability (intra-serial precision condition of measurement), measurement precision, trueness of the measurement, and reproducibility of the measurement, measurement bias and errors of the measurements were discussed.

► Forensic chemistry. ► Raman spectroscopy. ► Mass spectrometry. ► Natural products. ► Alkaloids.