Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1246459 | Talanta | 2006 | 6 Pages |
Abstract
Based on the multidomain structure of Pseudomonas aeruginosa exotoxin A, a fusion protein termed rPEA has been constructed, which is expected to serve as a gene carrier in vitro. The expression and purification of rPEA are described. The basal properties of rPEA as a gene carrier are evaluated by investigating its interaction with plasmid DNA and mimic biomembrane by surface plasmon resonance (SPR) and electrochemical methods. rPEA is proved to be able to bind with plasmid DNA with high affinity. It can also interact with lipid membrane and increase permeability of the membrane, so the probe molecules can easily reach the gold surface and exhibit the electrochemical response.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Aixue Li, Zhaozeng Sun, Ying Ma, Fan Yang, Hongsheng Ouyang, Xiurong Yang,