| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1246462 | Talanta | 2006 | 5 Pages |
Abstract
A competitive immunoassay for clenbuterol (CLB) based on capillary electrophoresis with chemiluminescence (CL) detection was established. The method was based on the competitive reaction of horseradish peroxidase (HRP)-labeled CLB (CLB-HRP) and free CLB with anti-CLB antiserum. The factors affecting the electrophoresis and CL detection were systematically investigated with HRP as a model sample. Under the optimal conditions, the tracer CLB-HRP and the immunoassay complex were separated, and the linear range and the detection limit (S/N = 3) for CLB were 5.0–40 nmol l−1 and 1.2 nmol l−1, respectively. The proposed method has been applied satisfactorily in the analysis of urine sample.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xinghu Ji, Zhike He, Xinping Ai, Hanxi Yang, Chuanlai Xu,