Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1246711 | Talanta | 2009 | 6 Pages |
Abstract
CE/Ru(bpy)32+ electrochemiluminescence (ECL) system with the assistance of ionic liquids (ILs) was successfully established for sensitive determination of verticine and verticinone in Bulbus Fritillariae for the first time. Migration behavior of alkaloid largely relies on the hydrogen bonding interactions between alkyl imidazolium cations in ILs and the alkaloids. Running buffer containing 40Â mmol/L 1-butyl-3-methylimidazolium tetrafluoroborate (BMImBF4) IL-8Â mmol/L phosphate resulted in significant changes in separation selectivity for alkaloids with similar structures. The highest sensitivity of the detection was obtained by maintaining the detection potential at 1.2Â V. Under the optimized conditions, relative standard derivations of the ECL intensity and the migration time were 3.27 and 2.84% for verticine and 4.42 and 1.69% for verticinone, respectively. The standard curves were linear between 1Â ÃÂ 10â8 and 1Â ÃÂ 10â6Â mol/L for verticine and between 5Â ÃÂ 10â8 and 1Â ÃÂ 10â6Â mol/L for verticinone, respectively. Detection limits of 1.25Â ÃÂ 10â10Â mol/L for verticine and 1Â ÃÂ 10â10Â mol/L for verticinone were obtained (S/NÂ =Â 3). Developed method was successfully applied to determine the amounts of alkaloids in Bulbus Fritillariae.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ying Gao, Yuanhong Xu, Bingyan Han, Jing Li, Qian Xiang,