Chiral separation of β-adrenergic blockers on CelluCoat column by HPLC

The chiral separation of 10 β-adrenergic blockers (acebutalol, alprenolol, bufuralol, bisoprolol, celiprolol, carazolol, indenolol, metoprolol, oxprenolol and propranolol) was achieved on CelluCoat column (250 mm × 4.6 mm, 5 μm particle size). The mobile phases used were (90:10:0.2, v/v/v) and (95:5:0.2, v/v/v) combinations of n-heptane–ethanol–diethylamine, respectively. The flow rates were 0.5, 1.0 and 2.0 mL min−1 with detection at 225 nm. The capacity (k), selectivity (α) and resolution (Rs) factors were 0.44–12.91, 1.12–2.19 and 1.00–9.50, respectively. The proposed supra-molecular models indicated that the chiral resolution were governed by π–π interactions, hydrogen bondings and steric effect.