Article ID Journal Published Year Pages File Type
1246986 Talanta 2009 7 Pages PDF
Abstract

The cloud point extraction (CPE) preconcentration of ultra-trace amount of mercury species prior to reverse-phase high performance liquid chromatography (HPLC) with inductively coupled plasma mass spectrometry (ICP-MS) detection was studied. Mercury species including methyl-, ethyl-, phenyl- and inorganic mercury were transformed into hydrophobic chelates by reaction with sodium diethyldithiocarbamate, and the hydrophobic chelates were extracted into a surfactant-rich phase of Triton X-114 upon heating in a water bath at 40 °C. Ethylmercury was found partially decomposed during the CPE process, and was not included in the developed method. Various experimental conditions affecting the CPE preconcentration, HPLC separation, and ICP-MS determination were optimized. Under the optimized conditions, detection limits of 13, 8 and 6 ng l−1 (as Hg) were achieved for MeHg+, PhHg+ and Hg2+, respectively. Seven determinations of a standard solution containing the three mercury species each at 0.5 ng ml−1 level produced relative standard deviations of 5.3, 2.3 and 4.4% for MeHg+, PhHg+ and Hg2+, respectively. The developed method was successfully applied for the determination of the three mercury species in environmental water samples and biological samples of human hair and ocean fish.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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