In situ chemical reductive growth of platinum nanoparticles on glass slide for the mass fabrication of biosensors

Platinum nanoparticles (PtNPs) attached to glass slide surface was successfully prepared by using a simple in situ chemical reduction method. In this method, a ∼10 nm gold layer was first sputtered uniformly onto the glass slide surface, PtNPs could be grown directly on the gold layer by immersing the glass slide into the grown solution containing H2PtCl4 and ascorbic acid. The gold layer sputtered uniformly serves as “seed” for the following growth of PtNPs and high dense of PtNP modified film can be prepared. Control experiment without the gold layer found no obvious formation of PtNPs indicating the importance of the “seed”. The electrocatalytic effect of the PtNP film was investigated with the detection of hydrogen peroxide and for the fabrication of biosensors. Glucose oxidase was selected and directly electrodeposited onto the PtNPs modified surface. The resulting biosensor has a fast response time (<10 s) with wide linear range (5 × 10−6 to 2 × 10−2). The fabrication method is simple, convenient and can be used for the mass fabrication of biosensors. The present preparation method of PtNPs modified film could be used for the preparation of other metal nanoparticle and find electrochemical applications as well as for optical uses.