| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1247157 | Talanta | 2008 | 4 Pages |
Abstract
We found that DNA probes can be immobilized on anodically prepared porous niobium oxide without a chemical modification of both the DNA probes and the substrate. By using the porous niobium oxide with a positive surface charge, DNA hybridization events are detected on the basis of the blue-shift of a maximum absorption peak in UV-vis-NIR spectroscopy. The blue-shift is ascribed to the change of surface charge upon single- or double-stranded DNA. The method does not require a label and shows high sensitivity with the detection limit of the concentration of 1Â nM.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jinsub Choi, Jae Hoon Lim, Sangchul Rho, Deokjin Jahng, Jaeyoung Lee, Kyung Ja Kim,