Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1247348 | Talanta | 2006 | 6 Pages |
Abstract
Peroxynitrite was one of the important reactive oxygen species (ROS) which have been focused for many years. Peroxynitrite is an extremely strong and reactive oxidant which can cause many diseases linked to inflammatory processes and autoimmune diabetes, etc. ROS can significantly react with peroxidase and nicotinamide adanine dinucleotide (NADH) which is linked to numerous biological processes. NADH, NAD and horseradish peroxidase (HRP) were included in peroxidase-oxidase (PO) oscillation, dissolved oxygen was concerned with the oscillation and many ROS intermediates came into being in PO oscillation which was sensitive to the perturbation of ROS. The influence of peroxynitrite (ONOOâ) on this oscillation system was investigated. It was found that the oscillation amplitude increased when the system was perturbed with peroxynitrite. There was a linear relationship between the increment ratio in the oscillation amplitude and perturbing peroxynitrite concentration in the range 2.50Â ÃÂ 10â8 to 1.56Â ÃÂ 10â6Â mol/L. And further experimental results revealed that amplitude increasing may be caused by the effect of peroxynitrite on HRP. Based on this phenomenon, a highly sensitive method for the determination of peroxynitrite was developed.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Hongmei Wang, Ru-xiu Cai, Zhixin Lin,