| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1248018 | TrAC Trends in Analytical Chemistry | 2013 | 11 Pages |
Abstract
•Quantifying biopharmaceutical proteins in complex biological matrices by LC-MS/MS.•Enrichment at the peptide level after proteolytic digestion.•Chemical derivatization of peptides may enhance ionization efficiency.•Sample-preparation aspects of quantifying biopharmaceutical proteins.•Automating the entire analytical procedure.
Part I of this review discusses sample-preparation aspects of quantifying biopharmaceutical proteins in complex biological matrices by LC-MS/MS with a focus on blood-derived body fluids. We conclude Part I with a short overview over options for automating the entire analytical procedure, which is indispensable for routine applications in pharmacokinetic and clinical studies.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Rainer Bischoff, Kees J. Bronsema, Nico C. van de Merbel, Kees J. Bronsema, Nico C. van de Merbel,