Time-resolved detection of luminescence for electrophoretic methods

Time-resolved luminescence has been employed as a method of detection for electrophoretic studies for nearly two decades. We discuss experiments conducted on capillary-zone electrophoresis, capillary-gel electrophoresis, slab-gel electrophoresis and microchip electrophoresis. We place emphasis on the variety of sample molecules, and applications in order to show the versatility of this technique.

► Electrophoresis experiments using time-resolved luminescence detection are reviewed. ► Solutions to theory, experiment, and instrument-based challenges are discussed. ► Non-lifetime modes of detection can reduce background and improve sensitivity. ► Lifetime modes of detection permit peak identification and multiplexing by lifetime. ► More relevant methods need to be developed using time-resolved detection.