Elimination of electrochemical interferences in glucose biosensors

As classical biosensing systems, amperometric glucose biosensors have been widely studied and developed since Clark and Lyons first proposed the concept of glucose-enzyme electrodes in 1962. Although glucose oxidase can selectively and specifically catalyze oxidation of glucose, amperometric detection can hardly distinguish the current generated by the enzymatic reaction from the currents generated by electroactive species in biological samples. Various methods have therefore been reported to improve the overall selectivity of enzyme-based biosensors. This review summarizes the methods developed for eliminating electrochemical interferences. We emphasize our efforts to improve the performance of biosensors.