Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1249375 | TrAC Trends in Analytical Chemistry | 2009 | 15 Pages |
Abstract
This critical review summarizes recent developments in highly sensitive, specific assays using nucleic-acid (NA)-affinity probes and fluorescence detection. We emphasize two groups of DNA and RNA probes (i.e. aptamers and molecular beacons) because of the increase in their bioanalytical applications. The affinity and the specificity of these NA probes combined with the diverse detection capability of fluorescence measurements (e.g., intensity, polarization, resonance-energy transfer and decay life-time) enable ultrasensitive assays for proteins, gene mutations, single-nucleotide polymorphisms and molecular-binding events.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Alevtina A. Goulko, Feng Li, X. Chris Le,