Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1250109 | Vibrational Spectroscopy | 2016 | 6 Pages |
Abstract
Raman spectroscopy was used to directly compare different abilities of monomeric ribo- and 2â²-deoxyribo guanosine 5â²-monophosphates to constitute G-quartets and their supramolecular self-assemblies stabilized by Na+ and K+ cations in pH-neutral or slightly basic aqueous solutions. Nucleotide concentration, stoichiometry and temperature was systematically varied to show that in the presence of Na+ cations, 5â²-dGMP remains unordered under the conditions when 5â²-rGMP already forms Na+-stabilized G-quartets and soluble ordered assemblies. On the contrary, in the presence of K+ ions both 5â²-GMPs readily form self-stacking G-quartets, although self-association of 5â²-rGMP is still greater than that of 5â²-dGMP. These results are supported by 1H and 31P NMR spectroscopy. The cation-sensitive effect of ribose versus 2â²-deoxyribose substitution on the formation of G-quartets and soluble aggregates from monomeric 5â²-guanosine monophosphates under physiological pH could be essential for full understanding of different structural polymorphism of RNA and DNA quadruplexes.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
KateÅina MudroÅová, Václav ÅÃmal, Peter MojzeÅ¡,