Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1250248 | Vibrational Spectroscopy | 2011 | 6 Pages |
Abstract
Raman spectroscopy is a powerful technique for studying cellular biochemistry. In fact, each toxic chemical induces biochemical changes related to the own action mechanism. In this investigation Raman microspectroscopy has been used, in correlation with atomic force microscopy images, to detect biochemical and structural damages occurring in cultured human cells as a consequence of deltamethrin exposure. Cultured human keratinocyte cells have been exposed at increasing concentrations of deltamethrin from 10â3Â M to 10â6Â M for 24Â h. A viability test indicated that the cytotoxic dose corresponds to exposure at deltamethrin solution for 24Â h with the chemical concentration between 10â4Â M and 2.5Â 10â4Â M. The compared analysis of Raman spectra and AFM images allows to state that an evident damage occurs in the plasmatic membrane and it is already detectable after exposure of keratinocytes at the lowest investigated deltamethrin concentration (10â6Â M). The most important modifications are related to the breakdown of CH2 bonds of lipidic chains, whereas proteineous bonds are less involved in the deltamethrin action. On the whole, cellular damage starts after exposure to deltamethrin doses well lower than that established as cytotoxic.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
G. Perna, M. Lasalvia, P. D'Antonio, G. Quartucci, V. Capozzi,