Article ID Journal Published Year Pages File Type
1250516 Vibrational Spectroscopy 2012 5 Pages PDF
Abstract

The Raman and surface-enhanced Raman scattering (SERS) spectra of the C-terminal peptide of pigeon cytochrome C (PCC87–104), were recorded. This peptide is widely used to study the immune response in vivo. Hydrophobicity and net charge parameters of PCC87–104, allowed prediction of the nature of its interaction with colloidal nanostructured silver surfaces. The SERS spectrum provided information about the organization and orientation of PCC87–104 on the surface of silver nanoparticles (AgNPs). The batch to batch reproducible SERS spectra were obtained by adding the colloidal AgNPs solution onto the dried analyte sample. On the basis of the SERS information and the analysis of the net charge of each amino acid residue in the peptide sequence, it is concluded that the interaction of the peptide and the AgNPs is mainly induced and oriented by the lysine residues. The spectroscopic results are supported by quantum chemical calculations, performed by using Extended Hückel theory for a model of PCC87–104 interacting with a silver surface.

► Net charge values and hydrophilic characteristics of the peptide PCC87–104 allowed to infer about your SERS activity. ► Lysine residues play an important role in the peptide–AgNPs interaction. ► Theoretical results confirm the inductive effect imposed by the lysine in the electrostatic peptide–AgNPs interaction.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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