Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1250593 | Vibrational Spectroscopy | 2011 | 4 Pages |
Abstract
It is the first report of direct, in situ detection of carotenoids at the subcellular level by using Raman microspectroscopy. Single crystals sequestered in a carrot cell were measured using FT-Raman spectrometer equipped with a microscope and 40× objective. The observed characteristic bands centered at 1518 cm−1 and 1156 cm−1 proved the crystals were composed of carotenoids with β-carotene being predominant. The obtained results show the potential of Raman microspectroscopy for identification and analysis of compounds localized in cytoplasm by taking measurements directly from a single plant cell.
Related Topics
Physical Sciences and Engineering
Chemistry
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Authors
Malgorzata Baranska, Rafal Baranski, Ewa Grzebelus, Maciej Roman,