Analysis of 17β-Estadiol from Sewage in Coastal Marine Environment by Surface Plasmon Resonance Technique*

In this study, surface plasmon resonance (SPR) for monitoring 17β-eatradiol (E2) was developed. The small molecule E2 was immobilized on a CM5 sensor chip for an indirect competitive immunoassay to detect E2. The SPR response based on the antigen-antibody reaction was measured by injecting the sample solution into the flow system. The limitation of detection was 0. 445 μg/L. The developed SPE-SPR system was applied to analyze the seawater samples. Recovery of E2 was 91. 6%-93. 1%. Relative standard deviations (RSD) for the E2 assay were between 10. 9%-15. 1% (n = 3). The range of determination of E2 samples from the sewage in the coastal marine environment was between ND (lower than detection limit) and ca. 11. 78 ng/L.