Purification and in vitro Activity of an Antimicrobial Peptide from Skin of Rana Temporaria Cheminensis, David

In this study, an antimicrobial component (RTCI) was purified from the skin of Rana temporaria chensinensis, Dauid. Antimicrobial activities of RTCI against clinical multi-drug resistant bacterial strains, including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureaus, Klebsiella oxytoca, Kkbsiella pneumonias, Enterococcus fae-calis, and Proteus mirabilis were measured in vitro by means of minimal inhibitory concentration and time-kill studies. The results indicate that RTCI could inhibit the growth of these bacteria at a proper concentration and suggest that RTCI shows a better antimicrobial activity to Gram-negative bacterial strains than to Gram-positive bacterial strains.