Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1251399 | Chemical Research in Chinese Universities | 2006 | 6 Pages |
Abstract
Germacrene A synthase(GAS) catalyzes the biosynthesis of germacrene A, which is a key precursor for sesquiterpene lactones. Cloning of a novel full-length cDNA encoding GAS from the medicinal plant Crepidiastrum sonchifolium (designated CsGAS) is reported in this study. The cDNA is 1837 bp long and contains a 1680-bp open reading frame encoding a 559 amino-acid protein. The functional expression of the cDNA in Escherichia coli, as an N-terminal thioredoxin fusion protein, with the pET32a vector yielding a recombinant enzyme. Sequence analysis was used to compare this enzyme with the mechanistically related epi-aristolochene synthase from tobacco, and the effect of possible involvement of a number of amino acids in sesquiterpene synthase on product specificity was also discussed.
Related Topics
Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
REN Jie, LIU Yan-qiu, YANG Li, NI Rui, YOU Song,