Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1251400 | Chemical Research in Chinese Universities | 2006 | 5 Pages |
Abstract
The cell adhesive motif RGD tripeptide was synthesized by using a novel chemical method. First, Gly-Asp(GD) was synthesized in two steps including the chloroacetylation of free L-aspartic acid and the ammonolysis of the chloro-acetylated L-aspartic acid. The yield of chloroacetylated L-aspartic acid was 83. 0%. For the ammonolysis of chloro-acetylated L-aspartic acid, the yield of the ammonolyzed product was 92. 3%. Second, the coupling between Arg and Gly-Asp was carried out by using the NCA method. The maximum yield of RGD was about 50% at 0 °C and pH = 9. 5. The prepared RGD tripeptide was confirmed by using amino acid component analysis and mass spectrographic analysis.
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Authors
WANG Hua, ZHAO Mi-feng, MA Cheng-yun, JING Wei, HUANG Yi-bing, HOU Rui-zhen, ZENG Hong-bin, XU Li, ZHANG Xue-zhong,