Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1251514 | Chemical Research in Chinese Universities | 2006 | 5 Pages |
Abstract
A sensitive, simple, and accurate method was developed for the determination and pharmacokinetic comparison of cinnamic acid in rat plasma after the administration of a Traditional Chinese Medicinal preparation, Di-Gu-Pi decoction, and pure cinnamic acid using RP-HPLC. Di-Gu-Pi was xtracted with 5% aqueous sodium bicarbonate, which was followed by purification with ion exchange column chromatography. The plasma samples taken from rats were deproteinized with methanol. The reversed-phase(HPLC) system with a Diamonsil C18 column and methanol-acetonitrile-water (8:32:60, volume ratio) (adjusted to pH =3.0 with glacial acetic acid) as the mobile phase was employed for the separation of cinnamic acid in the plasma samples. The detection was set at 272 nm and 3-(p-fluorophenyl)-2-propenoic acid was chosen as the internal standard. The calibration curve was linear in a range from 0.10 to 25.0 μg/mL (R2 = 0.9988, n = 9). The precision was 3.42%-10.10%; the between-day precision was 2.84%-8.91%; the accuracy was - 1.51% -1.26%; the mean recovery was 99.9%. The method was found to be sensitive, simple, accurate and appropriate for the determination of cinnamic acid.
Keywords
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Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Kang LI, Kai-shun BI,