Statistical Optimization of Medium Components for Improved Product Ion of Recombinant Hyperthermophilic Esterase1

The optimization of nutrient levels for the production of recombinant hyperthermophilic esterase by E. coli was carried out with response surface methodology (RSM) based on the central composite rotatable design (CCRD). A 24 central composite rotatable design was used to study the combined effect of the nutritional constituents like yeast extract, peptone, mineral salt and trace metals. The P-value of the coefficient for the linear effect of peptone concentration was 0. 0081 and trace metals solution was less than 0.0001, suggesting that these were the principal variables with significant effect on the hyperthermophilic esterase production. The predicted optimal hyperthermophilic esterase yield was 269. 17 U/mL, whereas an actual experimental value of 284. 58 U/mL was obtained.