A Repressor in 5′-UTR of hKv4.3 Gene1

The effect of repressors on ion channel gene expression was studied. The hKv4. 3 promoter and the sequence (+2— + 160, S160) of5′-UTR of the hKv4. 3 gene was cloned into the pB-gal-Basic vector. The transient expression of the pB-gal vector and the analysis of the relative activity of B-galactosidase were carried out. The analysis of the mRNA level was carried out with the RT-PCR method. S160 could intensively repress the expression of the hKv4. 3 gene with position-specificity. The level of mRNA did not alter obviously. A repressor (S160), in 5′-UTR of the hKv4. 3 gene was found and its repression to gene expression may play a role in the post-transcription process.