| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1251790 | Chemistry and Physics of Lipids | 2013 | 9 Pages |
•A new highly unsaturated cationic phospholipid is synthesized.•Its aggregation behavior, aggregate size and morphology are determined.•The lipid aggregate (liposome) forms with pDNA lipoplexes.•The lipoplexe formulated with DOPE and protamine shows in vitro transfection comparable to the standard reference Lipofectamine 2000.
The synthesis and self-assembling properties of a model compound in a new class of cationic phospholipids with a highly unsaturated conjugated fatty acid are described. In addition, the potential of this new lipid as a nucleic acid carrier was evaluated through lipoplex formulations employing 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) as helper lipid with and without the polycationic peptide protamine, together with a plasmid DNA (pDNA). Lipoplexes composed of this novel unsaturated lipid exhibited pDNA binding and protection from DNase I degradation when formulated with protamine. The new cationic lipid revealed transfection efficiency comparable to the commercial reference 1,2-dimyristoyl-sn-glycero-3-ethylphophocholine (EPC) in Chinese hamster ovary-K1 (CHO-K1) cells and performed equally to the standard reference Lipofectamine 2000 when the formulation included protamine.
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