Article ID Journal Published Year Pages File Type
1252068 Chemistry and Physics of Lipids 2010 4 Pages PDF
Abstract
The kinetics of the chain free-radical oxidation of cardiolipin (CL), a unique phospholipid containing four linoleate moieties, have been studied for the first time. The technique based on monitoring oxygen consumption by using a Clark electrode was applied to determine the oxidizability of CL during its initiated oxidation in aqueous Triton X-100 micelles in 50 mM phosphate buffer, pH 7.40, at 37 °C. The oxidizability was characterized by the k2/2k3 ratio, where k2 and k3 are the rate constants for the reaction of chain propagation (LO2 + LH → LOOH + L) and chain termination (2LO2 → products), correspondingly. The oxidizability of CL (in M−0.5 s−0.5) was found to be of 0.62 ± 0.07 (calculated on the basis of a single linoleate fragment) that is twice as much than the oxidizability of methyl linoleate (0.32 ± 0.04) determined in the same testing systems. It has been shown that the increase in k2/2k3 by a factor of two when going from the oxidation of ML to that of CL is almost completely determined by the increase in k2.
Related Topics
Physical Sciences and Engineering Chemistry Chemistry (General)
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