| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1252426 | Chemistry and Physics of Lipids | 2009 | 6 Pages |
Abstract
The effect of cytochrome c (cyt c) on degradation of cardiolipin in its polar part was investigated in cardiolipin/phosphatidylcholine (CL/PC) liposomes incubated with cyt c/H2O2/and (or) ascorbate by high-performance thin layer chromatography and MALDI-TOF mass spectrometry. It has been shown that phosphatidic acid (PA) and phosphatidylhydroxyacetone (PHA) were formed in the system under conditions where hydrogen peroxide favours a release of heme iron from cyt c. The formation of PA and PHA occurs via an OH-induced fragmentation taking place in the polar moiety of cardiolipin. Formation of fragmentation products correlated with the loss of CL in CL/PC liposomes incubated with cyt c/H2O2/ascorbate or with Cu2+/H2O2/ascorbate.
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Authors
Irina Yurkova, Dominik Huster, Juergen Arnhold,