Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1252967 | Chemical Research in Chinese Universities | 2008 | 5 Pages |
Abstract
A new adenosine biosensor based on aptamer probe is introduced in this article. An amino-labeled aptamer probe was immobilized on the gold electrode modified with an o-phenylenediamine electropolymerized film. When adenosine is bound specifically to the aptamer probe, the interface of the biosensor is changed, resulting in the decrement of the peak current. The response current is proportional to the amount of adenosine in sample. The used electrode can be easily regenerated in hot water. The proposed biosensor represents a linear response to adenosine over a concentration range of 1.0x10â7-1.0x10â4 mol/L with a detection limit of 1.0x10â8 mol/L. The presented biosensor exhibits a nice specificity towards adenosine. It offers a promising approach for adenosine assay due to its excellent electrochemical properties that are believed to be very attractive for electrochemical studies and electroanalytical applications.
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Authors
ZHENG Fan, WU Zai-sheng, ZHANG Song-bai, GUO Meng-meng, CHEN Chen-rui, SHEN Guo-li, YU Ru-qin,