Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1253164 | Chemical Research in Chinese Universities | 2008 | 4 Pages |
Abstract
Survivin, a novel member of inhibitor of apoptosis(IAP) protein family, is aberrantly expressed in cancer but undetectable in normal, differentiated adult tissues. The cancer-specific expression of survivin, coupled with its importance in inhibiting cell death and in regulating cell division makes it a useful diagnostic marker of cancer and a potential target for cancer treatment. Survivin cDNA amplified from the total RNA of 293 cells through RT-PCR was cloned into prokaryotic expression vector pRSET-B. The recombinant plasmid pRSET-B-Surv was expressed in E.coli BL21, and the relative molecule mass(Mr) of expressed fusion protein was approximately 21000. The recombinant protein was purified through Ni2+ affinity chromatography column and characterized by SDS-PAGE and Western blot. The purified recombinant protein was then injected into rabbits, and antisurvivin polyclonal antibody with a high titer was obtained.
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Authors
Hai-hong ZHANG, Xi-zhen ZHANG, Dong-hai ZHAO, He-liang SHI, Yong-hui YU, Yong-ge WU, Xiang-hui YU, Wei KONG,