A highly sensitive quantification of phytosterols through an inexpensive derivatization

A highly sensitive method for quantification of phytosterols based on HPLC has been developed by derivatization with the benzoyl chromophore. Introduction of the chromophore, benzoyl group, to phytosterols via simple and inexpensive derivatization greatly improved the UV response at 254 nm. Quantification of phytosterols was effectively performed by HPLC analysis with methyl benzoate as the internal standard after derivatization. This new method demonstrated outstanding yield of recovery (>95%) and excellent sensitivity (ng level) and was applicable for sterols from either plant or animal sources. This method is generally useful in phytosterol studies.

► Highly sensitive quantification of phytosterols with routine UV detector has been achieved. ► Derivatization was performed by inexpensive reagents and simple operation. ► Inexpensive internal standard method makes this method robust and practical.