Sortase-mediated ligations for the site-specific modification of proteins

•Sortase-mediated ligation is a versatile tool of protein chemistry.•Sortase-mediated ligation in comparison with other chemoselective ligation techniques.•Optimizing sortase-mediated ligation by substrate and enzyme engineering.

Sortase-mediated ligation (SML) is one of the most commonly used techniques for the site-specific modification of proteins. Here, a brief overview on advantages and limitations of this technology in comparison with other chemoselective protein modification techniques is provided and successful approaches that extend the application range of SML are discussed.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (173 K)Download as PowerPoint slide