Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1257348 | Current Opinion in Chemical Biology | 2009 | 8 Pages |
Abstract
Echinomycin, a bis-intercalator antitumor cyclic peptide, is biosynthesized by a unique nonribosomal peptide synthetase (NRPS). Successful heterologous expression of the whole gene cluster of echinomycin in Escherichia coli let us to investigate a further application of echinomycin NRPS. To construct a cyclic peptide library, our approach through both chemoenzymatic and rational genetic engineering has been successfully demonstrated. These achievements provided the further support that E. coli-based system can serve as a flexible yet robust platform for producing complex natural products and their analogs.
Related Topics
Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Kenji Watanabe, Hiroki Oguri, Hideaki Oikawa,