| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1257438 | Current Opinion in Chemical Biology | 2009 | 9 Pages |
Abstract
The (re)design of enzymes to catalyze ‘new’ reactions is a topic of considerable practical and intellectual interest. Directed evolution (random mutagenesis followed by screening/selection) has been used widely to identify novel biocatalysts. However, ‘rational’ approaches using either natural divergent evolution or computational predictions based on chemical principles have been less successful. This review summarizes recent progress in evolution-based and computation-based (re)design.
Related Topics
Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
John A Gerlt, Patricia C Babbitt,