DNA directed immobilization glycocluster array: applications and perspectives

•We review advances in DNA directed immobilization-based glycocluster array.•Glycocluster array is a tool for anti-pathogen molecule discovery.•Immobilization chemistry influences glycan/protein interactions in arrays.•The dissociation constant (Kd) and IC50 of glycoclusters targeting Pseudomonas aeruginosa virulence factors are determined with picomoles of molecules.

The present review concerns the recent advances in DNA directed immobilization (DDI) based glycocluster array. The impact of glycan immobilization on subsequent interactions with protein is discussed and the consequent pros and cons of DDI-based glycocluster array are reviewed. Finally, application in the discovery of anti-pathogen molecules is illustrated by screening for galactose or fucose glycoclusters targeting two Pseudomonas aeruginosa virulence factors (PA-IL and PA-IIL).