Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1266075 | Procedia Food Science | 2016 | 5 Pages |
A continuing goal in predictive microbiology is models directly based on physiological behavior. Buchanan et al.1 hypothesized that (1) the curvilinear lag/exponential transition represents the variability of cells in the adjustment (ta) and metabolic (tm) periods, and (2) the exponential/stationary transition is determined by limiting nutrient diffusion rates. Nutritional shift trials were conducted to estimate E.coli K-12 growth. Lactase production time suggest that lactase gene translation occurs after completion of lag phase. Agitation rates and inoculum sizes both influenced the shape of the exponential/stationary phase transition. Monte Carlo simulations allowed the generation of sigmoidal growth curves while considering physiological events.