Electrochemical evaluation of the interaction between antitumoral titanocene dichloride and biomolecules

Electrochemical investigation of the interaction between titanocene dichloride and several biomolecules, i.e., human and bovine serum albumin (HSA and BSA), and single-strand and double-strand deoxyribonucleic acid (ss- and ds-DNA) is reported. The addition of biomolecules to a hydroalcoholic solution of TiCp2Cl2 was followed by a decrease in the Ti(IV)/Ti(III) reduction peak current, due to the formation of adducts with lower diffusion coefficients. Qualitative analysis of the data reveals that titanocene dichloride has a higher affinity for proteins than for nucleic acids.

Graphical abstractElectrochemical investigation of the interaction between titanocene dichloride and several biomolecules (i.e., human and bovine serum albumin, and single- and double-strand DNA) is reported. The addition of biomolecules to a solution of TiCp2Cl2 was followed by a decrease in the Ti(IV)/Ti(III) reduction peak current, due to the formation of adducts with lower diffusion coefficients. Qualitative analysis of the data reveals that titanocene dichloride has a higher affinity for proteins than for nucleic acids.Figure optionsDownload full-size imageDownload as PowerPoint slide