Structural aspects of copper(II) binding by a multi-His analogue of somatostatin

•Coordination abilities of somatostatin analogue are characterized.•The impact of Cu(II) binding on the spatial arrangement of peptide is described.•The cyclic complex with the {4×NIm} binding mode dominates near to the pH 7.4.

In this paper we present the studies on coordination abilities of multi-His analogue of somatostatin. The somatostatin is a peptide hormone of which radionuclide-labeled analogues are successfully used in clinical practice in receptor scintigraphy. Its the analogue presented in this study is characterized by the presence of four His residues located in groups of two at both ends of the peptide. The -PheTrpLysThr- fragment placed between His residues is responsible for the spatial arrangement which determines the interaction with somatostatin receptors. In this paper we present the impact of copper ion binding on the spatial arrangement of the crucial fragment of peptide. The analysis of potentiometric and spectroscopic data allowed us to characterize the coordination abilities of the peptide and show that the ligand forms a {4×NIm} complex in the physiological range of pH. Results of the molecular modeling gave an insight into the structural aspects of this complex.

Graphical abstractThe proposed structure of the CuHL complex for Ac-HHPFWKTFPHH-NH2.Figure optionsDownload full-size imageDownload as PowerPoint slide