Isothermal titration calorimetry of metal ions binding to proteins: An overview of recent studies

Isothermal titration calorimetry (ITC) is a technique that is capable of quantifying the stoichiometry, equilibrium constants and thermodynamics of molecular binding events. Thus, important information about the interaction of metal ions with biological macromolecules can be obtained with ITC measurements. This review highlights many of the recent studies of metal ions binding to proteins that have used ITC to quantify the thermodynamics of metal–protein interactions.

Graphical abstractRecent studies where isothermal titration calorimetry (ITC) has been used to quantify the thermodynamics of metal ions binding to proteins are reviewed, including this example of Ni2+ binding to the urease metallochaperone protein UreE.Figure optionsDownload full-size imageDownload as PowerPoint slide