Article ID Journal Published Year Pages File Type
1310596 Inorganica Chimica Acta 2013 10 Pages PDF
Abstract

Bis[(1-methylpyrrolidin-2-yl)methoxy] [phthalocyaninato] silicon (2), bis[2-azepan-1-yl)ethoxy] [phthalocyaninato] silicon (3) and bis[(2,4,6-tris(N,N-dimethylamminomethyl) phenoxy)] [phthalocyaninato] silicon (4) were prepared and they were quaternized with the excess of iodomethane to obtain the compounds 2Q, 3Q and 4Q respectively. The binding of quaternized 2Q, 3Q and 4Q phthalocyanines (pcs) with calf thymus (CT) DNA was investigated by UV–Vis, fluorescence spectrophotometric methods and gel electrophoresis. The quenching effect of all quaternized pcs on the fluorescence intensity of SYBR Gold (SYBR)–DNA complex was determined. The results indicated that novel pcs exhibit efficient DNA binding activity. Photocleavage of CT-DNA in the presence of 2Q, 3Q and 4Q were determined using gel electrophoresis. All the experimental data proved that these pcs might be the candidates as DNA-targetting PDT agents.

Graphical abstractBy substituting three different functional groups axially to (1), 2, 3 and 4 were prepared. The binding of quaternized 2Q, 3Q and 4Q with calf thymus DNA was investigated by UV–Vis, fluorescence spectrophotometric methods and gel electrophoresis. Photocleavage of DNA in the presence of 2Q, 3Q and 4Q were proved.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Novel functional groups were substituted axially to silicon phthalocyanine. ► Interaction of quaternized phthalocyanines with DNA were examined. ► Photocleavage of DNA was observed.

Related Topics
Physical Sciences and Engineering Chemistry Inorganic Chemistry
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