Structural analysis of the anti-arthritic drug Auranofin: Its complexes with cysteine, selenocysteine and their fragmentation products

Positive mode electrospray mass spectrometry was used to investigate complexes of cysteine and selenocysteine with the metallo-drug Auranofin and the analogous chlorotriethylphosphine gold. Evidence for all the complexes, with the exception of the Auranofin–selenocysteine complex, was obtained. Several fragmentation products were examined and their proposed structures reported. Structural details of many experimentally observed ions including [Auranofin + H]+, [(CH3CH2)3P–Au–cysteine]+ and [(CH3CH2)3P–Au–methylselenocysteine]+ were examined by means of hybrid density functional theory at the B3LYP/DZVP level. Structural information and relative free energies are presented for several isomers of each ion considered.

Graphical abstractFormation and fragmentation of Auranofin complexes with cysteine and seleocysteine.Figure optionsDownload full-size imageDownload as PowerPoint slide