Differential speed of activation in antioxidant system in three oat genotypes

•The oxidative stress caused by Al toxicity can trigger root growth inhibition in oat.•The antioxidant system was activated after 12 hours in tolerant genotype.•The cellular redox status of oat genotypes seems to be affected by Al.•Oxidative stress may be important mechanism involved in Al tolerance.

The objective of this study was to evaluate whether the oxidative stress caused by aluminum (Al) toxicity is a symptom that can trigger root growth inhibition in oat genotype seedlings. Oat seedlings were grown in a nutrient solution (pH 4.0) with 0 and 370 μM Al. At 12, 24, and 36 h after Al addition, growth (root length) and biochemical parameters (catalase — CAT, ascorbate peroxidase — APX, and superoxide dismutase — SOD activities, lipid peroxidation, ascorbic acid (ASA) and non-protein thiol group (NPSH) concentration) were determined. The aluminum content was measured in oat seedlings. Regardless of the exposure time, root of the tolerant genotype grew normally with any Al treatments. Al supply caused lipid peroxidation only in the Al-sensitive genotype in roots and shoots (at 12, 24, and 36 h). In sensitive genotype seedlings, CAT, APX, and SOD were activated only at 24 or 36 h. In tolerant and intermediate genotypes, CAT, APX, and SOD were activated at 12, 24, and 36 h. Data for root growth and lipid peroxidation suggested that lipid peroxidation in the sensitive genotype may be an effect of Al toxicity on root growth. Therefore, the tolerant, intermediate, and sensitive genotypes differ in the expression of the amount, type of antioxidants, and speed of activation of antioxidant system, suggesting a varying capacity of these genotypes to deal with oxidative stress, which resulted in varying sensitivity and tolerance to Al.

Graphical abstractThe objective of this study was to evaluate whether the oxidative stress caused by aluminum is a symptom that can trigger root growth inhibition in oat genotype. The genotypes differ in type of antioxidants and speed of activation, suggesting a varying capacity of these genotypes to deal with oxidative stress.Figure optionsDownload full-size imageDownload as PowerPoint slide