Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1316216 | Journal of Inorganic Biochemistry | 2009 | 5 Pages |
Abstract
Lanthanide Eu3+ and Tb3+ ions have been widely used in luminescent resonance energy transfer (LRET) for bioassays to study metal binding microenvironments. We report here that Eu3+ or Tb3+ can increase the binding affinity of antitumor antibiotic drug agent, 7-amino actinomycin D (7AACTD), binding to 5′-GT/TG-5′ or 5′-GA/AG-5′ mismatched stem region of the single-stranded hairpin DNA. Further studies indicate that the effect of Eu3+ or Tb3+ on 7AACTD binding is related to DNA loop sequence. Our results will provide new insights into how metal ions can enhance antitumor agents binding to their targets.
Related Topics
Physical Sciences and Engineering
Chemistry
Inorganic Chemistry
Authors
Yinghua Peng, Yujun Song, Lingyan Feng, Jinsong Ren, Xiaogang Qu,