| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1317148 | Journal of Inorganic Biochemistry | 2007 | 7 Pages |
Abstract
The present study demonstrates direct electron transfer between cytochromes P450 2B4 (CYP2B4), P450 1A2 (CYP1A2), sterol 14α-demethylase (CYP51b1) on the one hand and screen-printed graphite electrodes, modified with gold nanoparticles and didodecyldimethylammonium bromide (DDAB) on the other. Electro detection of heme proteins was possible when 2–200 pmol P450/electrode were adsorbed on the surface of nanostructured electrochemical interfaces. Electron transfer, direct electrochemical reduction and interaction with P450 substrates (oxygen, benzphetamine, and lanosterol) and with P450 inhibitor (ketoconazole) were analyzed using cyclic voltammetry (CV), square wave voltammetry (SWV) differential pulse voltammetry (DPV), and amperometry.
Related Topics
Physical Sciences and Engineering
Chemistry
Inorganic Chemistry
Authors
V.V. Shumyantseva, T.V. Bulko, Yu.O. Rudakov, G.P. Kuznetsova, N.F. Samenkova, A.V. Lisitsa, I.I. Karuzina, A.I. Archakov,