Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1317638 | Journal of Inorganic Biochemistry | 2006 | 5 Pages |
Abstract
Cyclic voltammetry performed at rapid scan rates on cytochrome P450 from Pseudomonas putida (P450CAM) in didodecyldimethylammonium bromide (DDAB) films on graphite electrodes revealed a couple (E) at 830 mV (vs Ag/AgCl). E was not significantly observed at scan rates less than 30 V/s at room temperature, suggesting that the oxidized species is unstable. The lifetime of E could be prolonged at 4 °C, which allowed reversible access to E at scan rates as low as 1 V/s. E was found to be sensitive to imidazole in solution and to variations in pH, suggesting that the redox reaction is occurring at the metal center (i.e., FeIV/III). Electrolysis reactions with different P450 substrates revealed that the electrochemically generated high-valent species is able to convert thioanisole to methyl phenyl sulfoxide.
Related Topics
Physical Sciences and Engineering
Chemistry
Inorganic Chemistry
Authors
Andrew K. Udit, Michael G. Hill, Harry B. Gray,