Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1317778 | Journal of Inorganic Biochemistry | 2012 | 9 Pages |
This paper focuses on the synthesis, characterization and biological activity of new N,N′-methylene modified cyclohexyl ethylenediamine-N,N′-diacetate (edda)-type ligands and their Pt(IV) complexes. Both the ligands and complexes were characterized by infrared, UV–vis, ESI-MS, 1D (1H, 13C, 195Pt) and 2D (COSY, HSQC, HMBC) NMR spectroscopy and elemental analysis. The possible correlation between the reduction potentials and the cytotoxicity of the complexes was examined. The potential antitumoral activity of all compounds was tested in vitro on human melanoma A375, human glioblastoma U251, human prostate cancer PC3, human colon cancer HCT116, mouse melanoma B16 and mouse colon cancer CT26CL25 cells, as well as primary fibroblasts and keratinocytes. The results obtained revealed strong antitumor potential of the newly synthesized drugs with preserved efficacy against cisplatin resistant lines and less toxicity towards nonmalignant counterparts. The mechanism found to be responsible for the observed tumoricidal action of each synthesized compound was induction of apoptosis generally accompanied with caspase activation. Taken together, the effective response to the treatment of a wide range of different cell lines, including cisplatin resistant subclones, as well as induction of apoptosis, as the mechanism suggested to be the most desirable way of eliminating malignant cells, represents a great advantage of this novel group of drugs in comparison to other members in this metallo-drug family.
Graphical abstractThis paper focuses on synthesis and biological activity of a new N,N′-methylene cyclohexyl ethylenediamine-N,N′-diacetate-type of ligands and Pt(IV) complexes. Antitumoral activity was tested in vitro in several tumor cells as well as primary fibroblasts and keratinocytes. Results showed that compounds induced apoptosis, which is mainly mediated by caspase activation.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights►We synthesize modified cyclohexyl edda ligands and corresponding Pt(IV) complexes. ►Structure modifications lead to spectral changes and improved biological activity. ►Novel Pt(IV) complexes show higher cytotoxic activity than cisplatin. ►We confirmed that all compounds induce caspase-dependent apoptosis.