Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1317885 | Journal of Inorganic Biochemistry | 2011 | 6 Pages |
Copper(II) complexes of di-, tri- and tetra peptides with previously published protonation constants were re-investigated using pH and copper ion selective electrode (ISE) potentiometry in conjunction with a modified version of HYPERQUAD computer program. The purpose was to demonstrate the suitability of the ISE approach for the determination of apparent stability constants for copper(II) complexes with ligands for which proton stability constants were not available. The interactions of Cu2+ with oligopeptides were also analysed using surface enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI-ToF-MS). The results provide an insight into the metal complex species formed, their apparent stabilities under selected conditions and the effect of the relative positions of certain amino acids within the peptide sequence.
Graphical abstractDemonstration of the suitability of the Ion Selective Electrode (ISE) approach for the determination of apparent stability constants.Figure optionsDownload full-size imageDownload as PowerPoint slide