| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 13472060 | Food Chemistry | 2020 | 34 Pages |
Abstract
In this study, we produced a sensitive and specific monoclonal antibody (mAb) against diclazuril based on a new hapten. The mAb, which belongs to the IgG2 subclass, had a 50%-inhibitory concentration of 0.36â¯ng/mL. A cross reactivity test revealed that the mAb had good specificity for diclazuril. A gold nanoparticle-based lateral-flow strip was assembled for the rapid screening of diclazuril in raw chicken breast and thigh samples. The strip assay had a visual limit of detection (LOD) of 2â¯Î¼g/kg and a cut-off value of 20â¯Î¼g/kg for diclazuril in chicken samples when evaluated with the naked eye. With the aid of a strip scan reader, the proposed assay obtained a quantitative measurement for diclazuril with an LOD of 1.08â¯Î¼g/kg. Therefore, this gold nanoparticle-based lateral-flow strip assay represents a potentially useful tool for on-site detection and rapid initial screening of diclazuril in chicken samples.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Zhongxing Wang, Xiaoling Wu, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu,