Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1351887 | Biochemical Systematics and Ecology | 2010 | 7 Pages |
Abstract
Myosin light chain 2 (MLC-2) gene was isolated and characterized from Antheraea pernyi, a well-known wild silkmoth. The isolated cDNA sequence is 905Â bp in length with an open reading frame of 612Â bp encoding a polypeptide of 203 amino acids. Semi-quantitative RT-PCR analysis showed that the MLC-2 gene was transcribed during four developmental stages (egg, larva, pupa, and moth), and present in all tissues tested. Alignment analysis revealed that the deduced protein sequence has over 95% identity to myosin light chain 2 of lepidopteran species, and 57-88% identity to other insect species, suggesting that insect MLC-2 proteins are highly conserved throughout evolution. The protein sequence was used to construct phylogenetic trees with other known vertebrate and invertebrate MLC-2 sequences, and the obtained trees demonstrated similar topology with the classical systematics, indicating the potential value of MLC-2 gene in phylogenetic study.
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Authors
Lin Liu, Hui-Ying Wang, Hong-Yu Jin, Song Wu, Yu-Ping Li, Yan-Qun Liu, Xi-Sheng Li, Li Qin, Zhen-Dong Wang,